Neymar made his second appearance for Al Hilal as he continues his return following a year-long absence due to an ACL tear, but it didn't last long.
The Brazil international came on in the 58th minute of his team's 3-0 win in an Asian Champions League Elite match on Monday, but was subbed off less than 30 minutes later after going to ground and grabbing the back of his right leg.
Aleksandar Mitrovic scored a hat trick to lead Al Hilal to the win and put them at the top of the league phase table of the Asian Champions League
Neymar saw his first action for Al Hilal, who he joined in 2023 from Paris Saint-Germain, on Oct. 21, but hadn't played again until Wednesday's game against Esteghlal as he cannot be registered for Saudi Pro League games until January.
Al Hilal had planned a slow return for Brazil's all-time leading scorer, who suffered a cruciate knee ligament injury during Brazil's qualifier against Uruguay shortly after joining the Saudi side and underwent surgery last November.
Neymar was also left off Brazil's latest squad for a pair of November World Cup qualifiers, with coach Dorival saying the team and federation didn't want to rush his return to the national team.
Brazil are fourth in their World Cup qualifying group, four points adrift of leaders Argentina after 10 games.
Information from Reuters was used in this story.