The victories of the newly promoted teams, 9 de Octubre and Chacaritas Jrs., marked the first round of the series B championship. Super-9 won 1-0 at home against Manta and the auriazules also won by the same score against Gualaceo.

The series and the round started at the Alejandro Ponce Noboa stadium in Guayaquil, where Gualberto Caicedo scored the winning goal for 9 de Octubre at the 25th minute, ending their 22-year absence from the top flight. Manta came close to equalizing, but Ángel Ledesma missed a penalty kick at the 49th minute. This match was played without spectators following the confirmation of a positive coronavirus case in Guayaquil by the Ministry of Health.

Atlético Santo Domingo also secured a home victory. Tsáchila team defeated Santa Rita 3-0, with goals from Hancel Batalla at the 33rd minute, Javier Charcopa at the 67th minute, and Álex George completing the rout at the 85th minute.

In Cayambe, the two teams relegated from the top flight in the 2019 season faced each other. América club won 2-1 against Fuerza Amarilla. José Lugo (37th minute) opened the scoring for the cebollitas, while Yordan Ayoví equalized at the 42nd minute. However, Lugo (45th minute) scored again just before the end of the first half, securing the victory for América.

Atlético Porteño surprised Independiente Juniors, who changed their venue to the Rumiñahui stadium in Sangolquí for the 2020 season after playing their matches in Latacunga in 2019. A goal from Sergio Mina at the 15th minute was enough for the visitors to win.

At the Ciudad de Pelileo stadium, located 20 minutes from Ambato, Chacarita, an absolute newcomer to the top division of national football, achieved a 1-0 victory over Gualaceo.

The Azuayan team pressed from the beginning to open the scoring, but it was the efficiency of the home team that scored at the 7th minute with Darwin Núñez, taking advantage of a rebound from goalkeeper Robert Toloza.

Gualaceo sought the equalizer, but their lack of effectiveness in their attacks and the expulsion of Federico Flores hindered their offensive ambition.

The match ended with incidents on the field of play, which required police intervention. (D)